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The phrase "a first line of" is correct and usable in written English.
It can be used when referring to the initial part or opening statement of a text, speech, or any written work.
Example: "The author crafted a first line of the novel that immediately captured the reader's attention."
Alternatives: "an opening line of" or "a leading line of".
Exact(59)
Our native social hypocrisy is a first line of defense.
The airway epithelium generates reactive oxygen species (ROS) as a first line of defense.
Emission and laser based analysis techniques are becoming a first line of defense for device analysts.
Petroleum jelly, and Vaseline in particular, was often a first line of care.
A lender of last resort ought not to be a first line of defense.
And it is using this as a first line of defence.
And Athens was protected by four mountain systems offering a first line of defense.
Even so, some drugs I can no longer use as a first line of treatment.
Their loss would deprive the people of a first line of protection against improper executive conduct in appropriate cases.
Indeed, seat theory is designed as a first line of defense against corporations governed by (presumably less stringent) foreign laws.
Similar(1)
They focused on the innate immune system, a first-line of defense.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com