Sentence examples for a first antibody from inspiring English sources
Staining without applying a first antibody served as negative control.
Membranes were blocked with 5% (w/v) dried milk in 100 mmol/L Tris, pH 7.5, 0.1% (v/v) Tween 20 and 150 mmol/L NaCl (TBST) for 1 h prior to overnight incubation at 4°C with anti-CTGF, anti-phospho-Akt or anti-phospho-JNK (Cell Signaling, Danvers, USA) antibody as a first antibody.
Preimmune rabbit IgG was used as a first antibody control.
As a first antibody for APN/CD13 staining, anti-APN/CD13 mAb (Novocastra, CD13mAb clone: 38C12, Newcastle, United Kingdom) was used at a dilution of 1 100.
FLSs were stained with anti-LPA1 monoclonal antibody (mAb) (1G6; LSBio, Seattle, WA, USA) as a first antibody, and phycoerythrin-conjugated anti-mouse immunoglobulin G (IgG) antibody (BioLegend, San Diego, CA, USA) as a second antibody.
Finally, strong synergy will be obtained between our study and the MATCH study by Schonewille et al. In the latter study, logistical/cost/and benefit aspects of advanced matching after formation of a first antibody will be determined.
This includes the pharmacokinetically bioequivalent option of subcutaneous administration of trastuzumab, which is strongly preferred by the patients [ 22], and a first antibody-cytotoxic conjugate, emtansine, highly active after trastuzumab pre-treatment [ 23, 24].
The use of a second antibody against N1 offered much greater specificity and reliability than the previous biosensor protocol.
As the sample travels farther up the strip, a second antibody binds to the antibody-virus pair, and a dark line appears on the strip, indicating infection.
The assay consisted of spiking the anti-tetani sample directly onto the toxoid modified SPE, and then a second antibody, i.e. a HRP-labeled anti-immunoglobulin G, was deposited onto the biosensor.
Maackia amurensis lectin (MAL) was covalently immobilized on Au-poly (methylene blue) (Au-PMB) acting as the signal amplification components, which was used to recognize the α2, 3-sial-Gs specifically like a second antibody linked on Au-PMB.
