This second PCR was used as a final screen to verify the gene of interest was integrated at the ku70 locus.
Next, after manual removal of the chorions, cldnb EGFP embryos were arrayed manually into the 96-well plates (two embryos per well) in 200 μl of system water at 20 hpf, resulting in a final screening concentration of 10 µM.
Two researchers will carry out a final screening process of the full-text articles to select articles that reach all our eligibility criteria.
As a final screening, unassigned DNA (free of annotated genes or TEs) was aligned using BLASTX against the NCBI nonredundant database.
As a final screening step, the full texts of the remaining publications were independently assessed for inclusion by pairs of reviewers once more (NG, DSK, WGWB, AB, WD, ALF, CF, MGM).
Cell profiles were counted at a final screen magnification of × 1024 using a 2D counting frame and a simple, unbiased counting rule (Gundersen, 1977).
"Twenty-two yeago ago tomorrow we had our final screening of 'Clerks' here," he told the crowd, launching into a profanity-strewn story involving a blood-stained contract, potato skins and Harvey Weinstein.
For those lucky enough to be inside the Castro Theatre on Sunday, June 19 , 1994 the final screening of the 18th Frameline LGBT Film Festival was very much a night to remember.
24 Final screening of "Women In Film".
The $1,500 prize was announced Saturday afternoon at the final screening of the three-weekend festival at Lincoln Center.
