Sentence examples for a final round of pcr using from inspiring English sources

This product was ligated to itself and used as the template in a final round of PCR using BARKpn3 and BARHind5.

The coding regions of the transcriptome are then captured from this library using sequence-specific probes, then a final round of PCR amplification and second strand digestion occurs to create the final library.

A final round of PCR is then employed in both methods to amplify the target product using outside primers.

Subsamples from each well were then used in a second round of PCR using Abgene PCR master mix with a non-proofreading polymerase (reaction conditions were 35 cycles of 94°C for 1 min, 53.6°C for 1 min and 72°C for 3 min, with an initial denaturation phase at 95°C for 15 min and a final extension phase of 72°C for 20 minutes).

A final round of purification was performed using AMPure beads.

The first round of PCR used P1 and P2 primers.

These fragments were subjected to two rounds of PCR using Pfu polymerase (Stratagene).

A final round of voting was inconclusive.

Voting was anonymous throughout, with the final round of voting made using an electronic keypad system.

A second round of PCR was performed using the N- and C-terminal fragments as a template with the two outside primers previously mentioned (each at a final concentration of 1 μM).

A second round of PCR was conducted using the extracted DNA as a template.