The final material, meso-tetra(2,6-dichloro-3-sulfonatophenyl porphyrin (H2,6-dichloro-3-sulfonatophenyl porphyrinol–acetone solution with a final reaction yield of 75%.
PCR was carried out in a final reaction volume of 25 µl in 200 µL capacity thin wall PCR tube in Eppendorf Thermal Cycler.
The reaction was carried out in a final reaction volume of 20 µL using 200 µL capacity thin wall PCR tube.
All qRT-PCRs were performed in a final reaction mixture of 10 μL contained 5 μL qPCR Master Mix, 0.5 mM each of forward and reverse primers, and 0.5 μL cDNA templates (about 600 ng/μL).
The absorption of SnS2 with a final reaction time of 12 h is a little intensified and shows a slight red-shift, which is caused by a enlarged particle size.
The reaction was initiated by addition of 25 μM elastase substrate (N-methylsuccinyl-Ala Ala-Pro-Val-7-amino-methyl-coumarin, Calbiochem) iN-methylsuccinyl-Ala Ala-Pro-Val-7-amino-methyl-coumarin
Each PCR reaction mixture contained 5 µl of immunoprecipitated chromatin in a final reaction volume of 20 µl.
The reaction was started by the addition of iron (III) citrate to final concentrations between 1 µM and 100 µM to a final reaction volume of 100 µL.
All assays were conducted in triplicates with a final reaction volume of 200 µL and measured using a SPECTRAMax 190 (Molecular Devices) 96-well plate spectrophotometer.
At time zero, 2x stocks of pre-warmed enzyme or buffer-only control were combined with Ub-AMC to yield 500 nM GST-tagged enzyme and 500 nM Ub-AMC in a final reaction volume of 100 µl.
Reactions were performed with the same concentrations of reagents and cycling parameters described above, with a final reaction volume of 50 µl, except that the reaction mix was prepared in a single fraction rather than two.
