Sentence examples for a final quantification from inspiring English sources
As all the analysed markers are illegitimately expressed in haemopoietic cells (Chelly et al, 1989), we considered a semiquantitative approach using RT PCR followed by Southern blot analysis, with a final quantification of radioactive intensity.
Before the final quantification, a subjective scoring of cryoprecipitate was adopted: absence of cryoprecipitate was scored as "−" while cryoprecipitate <1%, ranging from 1 to 5% and >5%, was scored as "+," "++," and "+++," respectively.
Final quantification was carried out on an Agilent 2100 Bioanalyzer with 1 μl of library.
A gene-wise entropy computed from the probabilities is used as final quantification of DS.
Fluorescence was determined using an AB 7500 Real-Time PCR system (Applied Biosystems, Foster city, CA) and final quantification was performed using the comparative threshold (Ct) method as previously described [12].
Final quantification and quality control of total RNA samples were performed using a 2100 Bioanalyzer (Agilent Technologies).
For a concentrations in the part-per-million (ppm) range, a recovery between 80 and 110% is normally acceptable without correction; therefore, there was no quantitative correction applied to the final quantification of catechin and EGCG in the tea samples analyzed.
Each group used different methods, but ultimately relied on Western blots for final quantification.
All of these methods relied on Western blots for the final quantification step, but used different variations.
Final quantification was based on three independent experiments in which each treatment condition and RT primer setting were assessed in triplicate.
