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The maximum percentage yield of protein extracted was about 100% with a final purity of 73.04%.
Regarding studies with sole surfactant treatment, Kim et al. (2003) applied SDS to Ralstonia eutropha cells, but additional heating at 121°C and washing steps were required to remove proteins and achieve a final purity of 97%.
The results showed that the best performing ATPS was composed of 11.25 % PEG 10,000 (w/w)/11.25 % Na2SO4 (w/w) at pH 5. The IL-18BP was recovered up to 98%% in the PEG-rich phase with a final purity of 92 % (Rosa et al. 2010).
When the elution step was studied, the kind of elution and pH were considered in order to obtain a product with a final purity of 4.0 with a purification factor of 6.35, so that, at the end of the strategy, C-phycocyanin of analytical grade would be obtained.
The crude product was purified by chromatography on silica showing a final purity of 99% (checked by a GC-MS ; Shimatzu QP2010).
zeae SENP8), respectively and improved after size exclusion chromatography (Superdex75-prep, Amersham) to a final purity of 30 40% (elaD and elaDC313S) and>90% (G. zeae SENP8), as assessed by Coomassie staining.
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Results: BODIPY-C16 could be radio-labeled in a Lewis Acid assisted F-18/F-19 F-18/F-19 F-18/F-19n a rexchangee yield (66%, not coreactionfor decay) and a final pureasonable C18 Sep-Pak purification of more than 97%.
After dialysis and syringe filtration, the final yield was 132.4 mg HP-NAP per gram of cell paste from E. coli culture, and the final purity of HP-NAP achieved was 99.32% with a total recovery of 82.1%.
The final purity of 86 % of IL-18BP had recovered (Kornmann and Baer 2008).
Thus the final purity of the metallic material is of crucial importance for long-term use in SOFCs.
Final purity of PHA in water suspension obtained using GC analysis is 92.6%, with a nearly 90% recovery, thus concluding that this method is indeed a suitable alternative.
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