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A final proof for targeted alterations in a ZFN-treated sample comes from sequence analysis.
As a final proof for the role of Rack1 in autophagy, we have generated transgenic animals expressing Rack1 with a C-terminal FLAG-HA tag by using a DNA construct from the ongoing Drosophila proteomics project.
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After fermentation, the dough was punched, transferred to a greased metal baking plate, and incubated at room temperature for a final proof of 45 min.
The latter point is apparent from Table 2, where for a final proof of principle of our mathematical calculations, results of the analysis of our integration data set retrieved from two clinical SCID-X1 therapy trials [unpublished data] are given.
A few weeks later we had to check and correct a final proof of the paper.
A proof of each colour block was made before a final proof from the key block.
As a final proof of the presence of infiltrating HEK293-pmeLUC cells within the tumour masses, tumour homogenates were assayed for the presence of pmeLUC message (figure 3E).
We found strong evidence for a final proof that inner tubes of DWCNT are capable of exhibiting PL.
As a final proof that PBL1 is required for the MAMP-induced calcium elevation, we introduced a genomic DNA fragment encompassing the PBL1 gene locus into the pbl1 T-DNA mutant.
"This is a final proof point that confirms the uptick," he said.
Hence, although our own and published data support an important protective role for myocardial PKG I in pathological cardiac hypertrophy in vivo [ 45], a final proof is lacking.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com