Sentence examples for a final library of from inspiring English sources

If necessary, new ligations were performed to give a final library of approximately 106 clones: the titer was estimated on Chloramphenicol LB plates.

Each of these molecules was represented by up to 10 different conformations, generating a final library of 4316 high-energy state compounds for docking, termed the SUG-HEI library.

The 18 CAM selected libraries were assembled in amounts proportional to the natural distribution of CDR3 loop lengths in human antibodies to give a final library of more than a billion of clones.

Specifically, the starting material was assumed to be 100 ng of mRNA that then resulted in 500 ng of library with an insert size of 250bp in a volume of 400 uL for a final library concentration of 10 nM.

A final library consisting of 568,080 collapsed, unique reads (i.e., non-redundant, single genome-mappers) was used for piRNA cluster identification.

Transformation reactions were plated on an average of 10 agar plates (140 mm diameter) containing 100 μg/ml ampicillin (or 50 μg/ml kanamycin, see Supplementary file 1) and 34 μg/ml chloramphenicol such that each plate contained 200 1000 colonies, leading to a final library size of 2000 10,000 variants.

The above control experiments with high-quality cell line DNA indicated good overall performance of the bisulfite PCR pre-amplification assay in producing a final library, and therefore the utility of the assay using clinical FFPE DNA was then assessed.

The PCR product was purified using the MinElute PCR Purification Kit (QIAGEN), and the concentration of a final library was measured using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA).

The final library of Ves fragments was sequenced on a single Illumina HiSeq 2000 through a commercial core lab (fig. 1 H).

The final library of putative Myxobolus pendula transcripts was converted into a BLAST database against which genes of interest were searched.

The final library of purchased compounds contained 33 ligands predicted by A and 74 compounds predicted by B; 18 compounds were common to both A and B. The final criterion for the acquisition of the 89 compounds was commercial availability.