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The oldest member of the cast, the cantaora Herminia Borja, has a voice that could wake the dead; she's the singer Amaya turns to when she needs a final injection of energy to push her over the edge.
These received a final injection of 106 cells two and one day before the fusion.
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Rapamycin (LC Laboratories) stock was prepared at 25 mg/ml in DMSO (Sigma) and delivered to mice comprising both sexes daily via intraperitoneal injections of 10 mg/kg body weight with a final injection volume of 100 μl. 100 μl aliquots of stock solution were stored at −20°C.
Rescue strain construct concentrations are listed in Table 1 with each being co-injected with the pmyo-3::CFP marker (L4816; Fire Lab C. elegans Vector Kit, 1999 plate) (20 ng/µl) and pCR2.1 added to a final injection concentration of 100 ng/µl.
Animals were anesthetized with halothane and aseptically injected via the tail vein with 10 pmol AβOs or Dulbecco's PBS, in a final injection volume of 100 μl.
l-DOPA (l-3,4-dihydroxyphenylalanine methyl ester hydrochloride) and benserazide hydrochloride were purchased from Sigma Aldrich and freshly dissolved in a saline solution at a final injection volume of 5 ml/kg.
Litters comprising both sexes were injected with either DMSO (vehicle) or 5 mg rapamycin per kg body weight diluted with 1X PBS to a final injection volume of 100 μl.
It allows for the slow starving of a human, albeit pain-free, but not a humane, quick, final injection of barbiturates, as you would give your dog.
Accordingly, we performed an additional experiment in which cholinesterase was measured 2 hr after the final injection of the highest dose of diazinon, a time point corresponding to the peak of inhibition (Dam et al. 2000).
The final injection of the enzyme substrate results in an amperometric signal.
The theft of the viola gave me that final injection of courage to make the move.
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