Crystals were supplemented with either PEG 400 or 5/4 PO/OH to a final concentration of 35% (vol/vol) prior to vitrification by direct immersion in liquid nitrogen.
Glycerol was used as a cryoprotectant and incrementally added to the mother liquor to a final concentration of 30% before flash-freezing the crystals by immersion in liquid nitrogen.
Surfaces were sterilized in 70% ethanol for 1 h immediately following plasma treatment, followed by three sequential 5 s immersions in 70% ethanol, two sequential 5 s immersions in HEPES saline solution and a final 10 immersion in cell culture media prior to cell seeding.
David wants a final mad immersion in lust, which arrives, not to his surprise, with its attendant idiocies of jealousy and possessiveness.
She wants a connection to culture; David wants a final mad immersion in lust, which arrives, not to his surprise, with its attendant idiocies of jealousy and possessiveness.
This was the final immersion for the EMBA class that graduated this past weekend.
The stage works were also represented in the final Immersion concert, for which Clark Rundell conducted the BBCSO.
After a final summative assessment, students have a period of clinical immersion in preparation for practice.
To reach a thickness of approximately 100 nm, four immersion-heating cycles were required, with a final treatment at 400 °C for 1 h in air at atmospheric pressure.
The imaging was performed on a Nikon Eclipse (TE2000-E) inverted microscope with a 60× water immersion objective (numerical aperture 1.0) with an additional 1.5× magnification for a final magnification of 90×.
Although he kept one of these paintings with him all his life, he appears to have stopped work on both around the time of his final stylistic immersion, into Surrealism.
