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A field sampling was conducted based on a stratified random design.
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Field sampling was conducted in 2 years (2005 and 2009) according to the methods reported in [19].
Field sampling was conducted at 17 sites in West Africa Ivoryy Coast and Central Africa Equatorial Guineaeandnd Cameroon) between 1993 and 2001 (Figure 1B; Table S2).
Field sampling was conducted from mid-June to early July 2009 in nine European countries.
Field sampling was conducted in 2 years (2005 and 2009) according to the methods reported in [ 19].
A two-stage sampling was conducted.
In July 2005, eight months after the initial isolation of RCOL-1, more intensive sampling was conducted in the field where RCOL-1 was originally collected, and three other strains with morphology similar to RCOL-1 were found, although not at the original site of collection.
Sampling was conducted in a non-protected area, with the approval of the commune of Le Monêtier-les-Bains Monêtier-les-Bains Monêtier-les-Bains
Duplicate sampling was conducted on 10-12% of field samples (across sessions), and relative precision was calculated using differences in standard deviation between each pairs of the duplicates, divided by the overall mean concentration.
The actual sampling was conducted by an unnamed federal agency and reported to the EPA.
Blood sampling was conducted by vein cannula.
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