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Because studies of the modern deposits often focus on one or a few transects, it is difficult to determine if a certain transect is an "exception" or represents the overall distribution of deposits.
The resultant probability map, together with the probability graphs developed for a few transects across the transition, are able to map and describe (1) sharp, narrow ecotones (under five meters); (2) moderate ecotones that have a distinct band of transition (over a few hundred meters); and (3) complex ecotones that include slow transitions, interdigitated boundaries and outliers.
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This result did not seem to depend on whether many transects of short distance or few transects of long distance were used (data not shown).
Length frequencies within species were generally very similar between the two methods, though a higher proportion of larger individuals was recorded for a few species during transect surveys.
The species therefore spread out by local dispersal during the dry season across habitats, whereas its population was concentrated on fewer transects during the rainy season in both, intact and degraded habitat.
The CalCOFI data set is re-sampled here to show that a reduced sampling program one based on a few stations along a single transect (cf >50 stations along 6 transects for CalCOFI) or one based on a shorter time series can, within limits, obtain similar single-species and multivariate patterns of abundance.
Differential distributional patterns in species richness and species abundance along the climatic transect of a few hundred meters (the geology is identical on both slopes) reveals ecological (climatic) selection strong enough to override the mixing effects of migration and stochasticity.
A plot of the flight paths (Fig. 1) shows that the transects were highly similar except in a few places at either end where flights occasionally went off-course.
More species were retrieved when samples were spread over multiple transects than when they were located in only one or two transects, indicating that even at relatively small scales (a few kilometers) there is a turnover of ant species within the same type of habitat.
Moreover, close inspection of fine-scale transects suggests sharp geographic transitions between nuclear and mitochondrial gene pools over a few tens of kilometers (Additional file 1: Figure S1).
The transect survey results demonstrate the necessity of high sample density in this region of the continental shelf where fish aggregate patchily over scales of a few kilometers.
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