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He said that perhaps the authors had fished out a few oocytes, despite their efforts to exclude them, along with the germ-line cells, and that these oocytes could have been the origin of the infant mice produced later.
Bulging of cytoplasm and an enlarged zona pellucida envelope were observed in a few oocytes treated with the first wash.
Further, since any single couple may typically produce a few oocytes or embryos that are abnormal merely by chance, it is difficult to determine whether sibling embryos as a group, or an embryo cohort, is "normal".
After the animals had produced a few oocytes, the clear structure of the spermatheca was first observed and the spermatids moved into the spermatheca.
Intersexuality in the fish ranged from those with a few oocytes within the testicular tissue (index = 1 2) to those fish with predominantly female tissue scattered within small patches of testicular tissue (index 5 6).
Ovaries of boule dsRNA-injected females possessed only a few oocytes at early stages of oogenesis, and few or no mature oocytes (not shown), indicating a requirement for boule in the progression of oogenesis.
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First, we noticed that disassembly of oocyte granules was incomplete in meg-3 meg-4 zygotes (Video 2, Figure 1 figure supplement 1), causing a few oocyte granules to persist through the first mitotic division (arrows in Figure 4A).
Most of these intersex fish (13 of 15) were mildly feminized (had a low intersex index, with few oocytes in their testes).
It has been estimated that approximately 12,000 oocytes are present in the hen ovary when sexually mature, but only a few hundred oocytes are selected to reach maturity and ovulate.
One possible explanation is that our work queried very young adult gonads with only a few mature oocytes, whereas Reinke et al. (2004) used older adults with many mature oocytes.
During primordial follicle formation, large cysts (a special cluster of oocytes) have been proposed to break into smaller cysts, and this process is repeated until a few individual oocytes remain [7].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com