This is achieved by an alternation of fast rotational head shifts and intersaccadic periods where head rotations are minimal.
Second, we propose a new method for fast rotational matching of subtomograms in real space.
In addition, a recently developed 2D fast rotational matching (FRM2D) algorithm (Cong et al, 2003, 2005; Cong and Ludtke, 2010), available in EMAN 1.8+, was adopted in the final refinement.
Moreover, we also propose a fast alignment method using fast rotational matching in real space.
To achieve this goal, we first identify a keypoint in the target complex whose relative location is invariant to rigid transformations and serves as the center of rotation in the fast rotational matching of the two subtomograms.
We further detect the rigid transform invariant center of mass of the target complex and propose an improved fast alignment method using fast rotational matching in real space.
The existence of a fast NCO rotational equilibrium between the boat conformations B (I) and B (II) has also been observed.
Here, we devise an improved alignment method, which formulates the fast rotational matching of subtomograms in real space.
The fast rotational alignment is based on sampling of simultaneously over all rotations R. To do so, we apply a fast 3D volumetric rotational matching (Kovacs and Wriggers, 2002; Xu et al., 2012).
Additionally, the fast rotational motion of the CF3- group in tfmF leads to a sharp single peak, which makes the tfmF-19F NMR method ideal for protein complex studies in high crowding conditions, such as cell lysate or cellular environment.
