For MD, the context of the run (i.e. whether it is a minimization, an equilibration, or a production run) was missing.
After focusing, the strips were first equilibrated in an equilibration buffer (6 M urea, 30% glycerol, 2% SDS, 50 mM Tris-HCl, pH 8.8) containing 65 mM DTT, and then in an equilibration buffer containing 53 mM iodoacetamide.
For measurements performed over a long period (of hours), we suggest that there should be an equilibration period (1 2 hours) and a lower flow rate.
The analysis time for each sample was 7 min, including a short column wash and an equilibration step.
The three slightly different conformations were subjected to an equilibration protocol through which a stable conformation of the hexasaccharide substrates was determined.
Ellipticity at that wavelength (218 nm) was monitored in 1 s intervals during a 60 s scan, with an equilibration time of 10 min prior to the scan.
The experiment consisted of an equilibration period of 6 days and a consecutive control period of 6 days followed by an experimental period of 24 h.
The principle of SPME is the partitioning of analytes from the sample to a coated fiber in the course of an equilibration.
The temperature is slowly increased to bring about sublimation typically at a ramp rate of 1°C/min followed by an equilibration time.
By controlling all the parameters, complete dye mineralization can be achieved within an equilibration time frame of 40 min, using a mere 100 mg of the photocatalyst material.
