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Schul et al. [57] revealed the pause duration separating pairs of syllables in the songs of T. viridissima as a critical song parameter for species recognition.
A rigorous statistical methodology has been defined for the identification of the beginning of the triggering event, based on the critical duration, i.e. the minimum dry period duration separating two stochastically independent rainy periods.
The minimum silence duration separating two utterances was set to 0.5 s like in NIST RT '04[11], since pauses smaller than this value were not considered to be segmentation breaks in a speaker's speech (it is also complementary to the scoring collar discussed later).
In contrast, the average pause duration separating pairs of syllables was only slightly longer at 21°C compared to 28°C (22 ms vs. 18 ms).
We chose to analyse according to comparator (placebo or active) and trial duration, separating short-term trials of less than six weeks, from those of six to 12 weeks.
An advertisement call from the type locality shows a green toad trill (similar to B. viridis; reminiscent of a canary trill) consisting of a series of up to 75 actively pulsed notes with a constant duration separated by constant internote intervals.
All syllables were 145 ms in duration, separated with a 75 ms silent gap.
After the fixation display that lasted 1000 ms duration, there were two stimulus intervals of 150 ms duration, separated by a blank display of 350 ms. The contrast increment was present in only one of these intervals, randomly selected.
To test if the phenotype of diminished adaptation in eNOS-deficient mice is also detectable with longer interstimulus intervals we stimulated the OE with two, longer odorant pulses (2 s duration) separated by a long interstimulus interval (28 s).
It was split evenly into 2 runs of equal duration separated by a short break.
The treatment and placebo intervals were 2 weeks in duration separated by a 4-week washout period.
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