The mosquitoes were dissected in a drop of ice-cold sterile DEPC-treated water and midgut from 25 30 mosquitoes was pooled in 100 µl DEPC treated PBS buffer at −70°C.
After applying a drop of ice-cold hemolymph-like saline solution, the larvae were cut open along the dorsal midline.
Migratory cells were isolated with the in vivo invasion assay, and after collection, they were extracted from the microneedles in a drop of ice-cold PBS on glass slides.
Dry coverslips were coated with a thin layer of FITC-conjugated gelatin and immediately overturned on a drop of 0.5% ice-cold glutaraldehyde in PBS for 15 min incubation in the dark.
It was the place where I learned to ski when I was 12 years old, coaxed by an outdoorsy cousin onto a trail that looked, to my novice eyes, like a sheer drop of ice and snow.
Using a Hamilton syringe to ensure accuracy, a 20 μL drop of ice cold saline with 100 μM sodium C-formate was placed on the group of larvae, and sharp forceps were used to tear the cuticle of each larva, releasing the hemolymph into the droplet, but without damaging any internal tissues.
"They are more about this ongoing discovery of all the ways electric light and candlelight can be transmuted through clear crystal — this mysterious material which can look like a drop of water or a block of ice".
Live it fully and experience it for all of its glorious blessings, to the very last ray of sunshine and the very last drop of ice cream.
2. Turn out onto counter or cutting board and knead by hand, adding a few drops of ice water if necessary.
Despite Hawaii's tropical location, during several past ice ages a drop of only a degree in temperature allowed snow to remain at the mountain's summit through summer, triggering the formation of an ice cap.
Add a drop of tea tree oil to an ice cold compress of witch hazel.
