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A double transformation of empty vectors served as negative control.
Positive control was a double transformation of pUT18C_zip and pKT25_zip (Euromedex), encoding an interacting leucine zipper.
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But, in this late summer, there has been a double transformation.
China is experiencing a double transformation in demography and family patterns.
The deletion of comI had a beneficial effect on the transformability, as CM1 displayed a doubled transformation frequency of 201% ± 4.6, an effect that is nevertheless 50-fold lower than the effect observed in B. subtilis NCIB3610, in which the deletion of comI resulted in an approximately 100-fold increase of the strain's transformability (Konkol et al. 2013).
The combinations of double transformation of the four CrSGD BiFC constructs always showed a specific nuclear fluorescence signal suggesting that CrSGD was able to at least dimerise irrespective to the accessibility of its extremities.
For the double transformation of the H 6-GFP-BD-CVIL line with the SV40-mRFP gene construct, 20 appearing calli were screened, and seven out of them showed strong fluorescence in the first generation of cell suspension cultures (35%).
A double logarithmic transformation of the data presented in Fig. 11 was added as an insert.
We will examine double sequence to double sequence transformation of independent identically distribution random variables with respect to four-dimensional summability matrix methods.
The p n p double transformation and ultrasensitive insensitive gas sensing of reduced-graphene-oxide-flake stacked films (rGOFSFs) were controlled by changing the annealing temperature from 100 to 400 °C on a sodium silicate substrate, as a result of the competition between the thermal reduction, decomposition, and diffusion of Na, as determined from comprehensive microstructure characterization.
To assess the robustness of our results, a leave one out sensitivity analysis of the pooled estimates of relative sensitivity and specificity, disease prevalence, and tests' positivity rate were pooled with the double arcsine transformation of Freeman-Tukey.
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