Your English writing platform
Discover LudwigExact(3)
Sedimentation velocity experiments were performed in a Beckman XL-A analytical ultracentrifuge using a double sector charcoal-Epon cell at 20°C and 40,000 rpm.
Sedimentation velocity experiments were performed in a Beckman XL-I analytical ultracentrifuge using a double sector charcoal-Epon cell at 20°C and 42000 rpm.
400µl protein was loaded into a double sector Epon cell with sapphire windows and centrifuged at 50,000rpm (182 000rcf at cell centre and 201 600rcf at cell bottom) at 20°C using an An-50Ti rotor (Beckman Coulter).
Similar(57)
A 400 μL aliquot was loaded into the sample compartment of a double-sector cell, assembled with a 1.2 cm charcoal-Epon centerpiece and quartz windows.
Sedimentation velocity experiments, using absorbance optics, were conducted by loading 10 μM Fas DD WT or 10 μM Fas DD V254N mutant samples (380 μL) and the protein dialysate (400 μL) into a double-sector Epon charcoal-filled centerpiece.
ClpP was analysed at a protein concentration of 10 µM in 50 mM Tris pH 8, 150 mM NaCl, 1 mM MgCl2, 0.1 M arginine in the presence of varying concentrations of ADEP2 using a Beckman Optima XL-A analytical ultracentrifuge and an An50Ti rotor equipped with double sector cells.
Analytical ultracentrifuge analysis was carried out on a Beckman XL-I ultracentrifuge using an An-55 aluminium rotor and double sector aluminium-filled Epon centrepieces.
Proteins samples (full-length, un-tagged) at concentrations of 1 3 mg/mL in 50 mM Tris HCl, 250 mM KCl, 1 mM EDTA, 10% (v/v) glycerol, pH 7.5) were centrifuged at 50 000 rpm at 20 °C using 12 mm double sector cells in an An60Ti rotor, while monitoring interference in a Beckman Optima XL-I analytical ultracentrifuge.
Sedimentation velocity experiments were run at 30,000 RPM (22°C) on an Optima XLI (Beckman-Coulter, Fullerton, CA), with a 4-hole An60Ti rotor using double sector cells with charcoal-filled Epon centerpieces (path length 1.2 cm) and sapphire windows.
This is reflected in an increasing degree of orientation to double sector employment after graduation.
Briefly, 400 μL samples of protein dissolved in phosphate buffered saline (PBS) to final concentrations between 50 and 500 nM were loaded in cell assemblies with standard 12 mm double sector centerpieces, inserted in a eight-hole rotor, and placed in the chamber of the ultracentrifuge which was evacuated to high vacuum (zero or a few micron pressure).
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com