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Th-gal4-driven SybGFP showed a dotted distribution within the VG and largely mimicked the mCD8GFP expression pattern.
Interestingly, when the Na4vSS values of inner membrane proteins were plotted as a dotted distribution, the existence of two protein groups become evident: a first group with an aggregation propensity similar to that of cytosolic proteins and a second group with particularly high Na4vSS values (Figure 10).
As expected, most COS7 cells expressing wild type HIPK2 showed a dotted distribution in the nucleus, with about 12.5% of the cells also exhibiting cytoplasmic GFP fluorescence.
After meiosis, we observed a dotted distribution of the methyltransferase in early and late canoe stage nuclei (Fig. 4A′, arrows); late canoe stage is the stage in which histones are replaced by protamines (Fig. 4A″).
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As expected [47], EGFP-HIPK2 showed a prevalent nuclear, dotted distribution and, to a lower extent, a nuclear/cytoplasmic, dotted localization (Fig. 6A and B).
The reflective boundary condition enables a precise control of the dot density within each cell, and is instrumental in achieving a dot distribution with both a high dot density variation and a high spatial uniformity.
Several MD computational techniques are integrated with the conventional MD scheme to enable the adjustment of the dot density in specific regions of the light guide in order to create a dot distribution with a high dot density variation and a high spatial uniformity.
Moreover, 24 h post-TGF β treatment p62 was mainly found with a dot distribution, which suggests an autophagosomal localisation.
Note that in the model of Gaussian dots distribution, a single distinctly explicit maximum of absorption is observed.
Treatment of HEK wild-type cells with geneticin for 24 h increased immunostaining against phosphorylated eukaryotic initiation factor 2 alpha (p-eIF2 α) in a dotted cytosolic distribution consistent with the formation of stress granules.
Figure 3 The Ge dots distribution by top-view SEM.
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