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To determine a possible concentration threshold for PAßN effect on cefoxitin MIC in the presence of cloxacillin, a dose assay was carried out with increasing PAßN concentrations and two different cloxacillin concentrations.
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As a preliminary means of initially identifying extracts with activity, the effects of treatment were evaluated in vitro, in a two dose assay testing lower and upper concentrations of 20 and 200 μg/ml against human carcinoma cell lines.
Flow cytometry was used to quantify the cellular uptake of CB[7]4 using a dose titration assay and a time course assay.
In a dose response assay, NSC 37408 (6,7-dihydroxy-1-benzofuran-3-one), our best compound, exhibited an IC50 of 3 μM in the presence of 100 nM copper.
To observe dose-dependent effects, we first performed a dose gradient assay in vivo.
The required dose was calculated from a dose response assay performed prior to the experiment.
The observation was further confirmed by a dose response assay, in which the IC50 was established.
To confirm these observations, we compared the efficacy of CTL activation between survivin-2B80-88 peptide and Hsp90 survivin-2B75-93 precursor peptide complex in a dose titration assay (Fig. 1b).
In a dose titration assay, Mo-DCs (1 × 10) were loaded with various doses of survivin-2B80-88 peptide or Hsp90 precursor peptide (survivin-2B75-93) complex for 2 h in 100 μL Opti-MEM and fixed with 0.01% glutaraldehyde.
Those two records should match explicitly and should refer to the actual injection time and not the dose assay time.
A dose-response assay indicated that concentrations of TSP-1 between 250 ng/ml to 1250 ng/ml produced a gradual and marked increase in the number of dendritic spines in pure neuronal cultures (Figure 4C).
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