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Each of the four A domains from each module of tetramodular ChNPS4 groups with strong support with the corresponding A domains of tetramodular AbNPS1 in the closely related Dothideomycete, A. brassicae.
However, A domains from NRPSs found in other euascomycetes that group with each of the ChNPS4 modules contain from two to six modules.
Fungal A domains from NRPSs with known function and/or chemical products present in GenBank were also included (Additional file 10).
A domains from FgNPS1, AnsidC, and Umfer3 module 2, have HIS at 278, and their cavities are quite hydrophilic and lined by similar sets of residues (Table 2).
Primers Microcystis-mcyC-F and Microcystis-mcyC-R were used in [ 13] Amino acid sequences of A domains from all genera were aligned using ClustalW [ 36].
All eleven A domains from this protein group together as a single well supported monophyletic group (Fig. 2) which also includes certain A domains from other fungal NRPSs, such as ChNPS1 module 2 and ChNPS3 modules 2 and 4. Peptaibol synthetases illustrate a more complex process of tandem duplication of modules of an NRPS.
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The contact between the upper and lower layer subunits could be divided into two areas: one involves the A domain from the upper layer subunit and the B domain from the lower layer subunit (the right side in Figure 4), while the other involves the A domain from the lower layer subunit.
This observation indicates that the loss of the A domain from CAO resulted in an inactivation of 3, 8-divinyl protochlorophyllide a 8-vinyl reductase [ 16].
The unrooted ML phylogenies of A and C domains from A. nidulans SidC are shown in Fig. 6Bi, Bii.
A-domains from T. ophioglossoides are highlighted in blue.
Using whole genome data of the sampled species of Trichoderma, A-domains from the Tr.
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