The phrase "a distinct sequence was" is correct and usable in written English.
It can be used when describing a specific order or arrangement of events, items, or elements in a context such as research, analysis, or storytelling.
Example: "In the experiment, a distinct sequence was observed in the behavior of the subjects, indicating a clear pattern."
Alternatives: "a unique order was" or "a specific arrangement was".
Sequences corresponding to E. coli 16S rDNA positions 300-514 wextractedcted from the multiple alignment and each distinct sequence was assigned as a 100%-ID phylotype.
To estimate the level of gene expression, the number of reads mapped to each distinct sequence was extracted.
In E. coli, there has been a major expansion in Clade I rhs through transposition to novel loci, and loss of other clades relative to other Escherichia spp. Comparison of C-terminal tips and dissociated fragments shows that while C-termini vary greatly within a locus, each distinct sequence is conserved in related strains, indeed in other species and genera.
A total of 33,384 (49.27% of all distinct sequences) and 31,001 (45.76% of all distinct sequences) were obtained as significant hits using the respective databases (Additional file 2: Table S2).
Due to the lack of T. chinensis genome information and the relatively short length of distinct gene sequences, only 25,812 unigenes (55.41% of all distinct sequences) were shown to be annotated with NR (Additional file 3).
The 16S alignment with 237,882 distinct sequences was taken from GreenGenes [33] (http://greengenes.lbl.gov).lbl.gov
A total of 52,571 distinct sequences were identified from the raw sequences, of which only 13,782 mapped to the human genome reference sequences.
A total of 59 distinct sequences were found in the complete mitochondrial control region in the 214 Iberian Roma analyzed (Table S1), out of which only two were shared between Portuguese and Spanish subjects (Hap38 and Hap49).
A total of 24,258 distinct sequences were obtained, of which 11,864 had microsatellite motifs.
After removing the partial overlapping sequences, a total of 118,218 distinct sequences were obtained (All-Unigene, mean size: 506 bp, N50: 611 bp) (Additional file 1).
