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When a disk unit is selected (Fig. 1A), the underlying genes and structural variation events are shown on the bottom panel, along with the chromosome with cytobands and transcripts with their exonic regions coloured in dark grey (Fig. 1B).
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The microscope is configured with a spinning disk unit to perform both confocal and wide-field fluorescence microscopy.
Confocal images were acquired on Zeiss Axio Observer Z1 equipped with a Yokogawa spinning disk unit and an EM-CCD camera.
Simultaneous visualization of mitochondria and actin filaments was performed on a spinning-disk confocal microscopy system consisting of a CSU10 spinning disk unit (Yokogawa Electric corporation) and an iXon EMCCD camera device (Andor, Belfast, Northern Ireland), mounted on a IX71 inverted microscope (Olympus, Japan).
Live cell observations of granulocytes during changes between normotonic (live cell medium) and hypotonic conditions (0.3× PBS) were performed on an Axio Observer D1 (Zeiss) with an UltraView VoX spinning disk unit (PerkinElmer) and an 63×/1.4 plan-apochromat oil objective.
Images (1024 × 1024 × 8 bit) were acquired on a Leica TCS SP5 AOBS confocal laser scanning microscope (Leica Microsystems Mannheim, Germany), using a one airy disk unit pinhole diameter and an HCX PL APO 20x/0.70 objective; magnifications were obtained by scan field selection.
The Ti-E-PFS system is outfitted with a Yokogawa CSU-X1 spinning disk unit, a self-contained four-line laser module (excitation at 405, 488, 561, and 640 nm), and Andor iXon 897 EMCCD camera.
Images of live cells were collected using a Cooke Sensicam QE CCD camera (Romulus, MI) mounted on an Olympus BX61WI microscope equipped with an Olympus confocal spinning disk unit (Melville, NY).
Cells were imaged using a spinning disk confocal microscope (Zeiss, Thornwood, NY, USA), equipped with a CSU-X1A 5000 spinning disk unit (Yokogowa Electric Corporation, Tokyo, Japan), multilaser module with wavelengths of 458, 488, 514 and 561 nm, and an Axio Observer Z1 motorized inverted microscope equipped with a precision motorized XY stage (Carl Zeiss MicroImaging, Thornwood, NY, USA).
The disk unit experienced a 4 ALR overall (INF-PRECHL).
The disk unit had an additional sample collection point that allowed the removal capacity of the isolated foam disk to be examined.
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