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Both a normal model and a diseased model with BO obstruction were developed for experimentation.
The peak velocity of pressure change (dp/dt) is a reliable index used to measure ventricular function, because parameters such as afterload, wall motion abnormalities and variations in ventricular anatomy and morphology that appear in a diseased model do not affect the results.
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Finally, to examine whether hPSC-derived EPs have the efficacy to promote vasculo/angiogenesis and to improve the organ function in a mouse diseased model such as MI, we injected 1 × 10 EPs directly into ischemic (peri-infarct) regions of myocardium immediately after ligation of the left anterior descending coronary artery (LAD).
The goal is to re-parameterize the diseased model to obtain a therapeutically treated model that, with a diseased input u tot,d, allows recovery from the diseased output to the healthy one, even in presence of uncertainty.
Improvements in oral exposure by a DCU nanosuspension formulation enabled a dose-dependent efficacy study in a diseased animal model [14].
The results demonstrated that the diseased model produced abnormal wrist extension and stress distribution as compared to the healthy wrist model.
We have identified the role of the suppressor of cytokine proteins (SOCS) family in combating detrimental effects of pro-inflammatory cytokines in degenerated human NP tissue as predicted by the developed diseased model.
As A, B, C, and D depend on the parameter values, they are different for each therapeutically-treated diseased model and for the healthy one.
These data provide direct experimental evidence that the EPs generated using the protocol described herein form functional vessel networks that anastomose with vessels of the host following transplantation with beneficial outcomes, even in the diseased model.
By providing various biochemical and biomechanical cues, healthy and diseased models at the organ level can be applied to disease progression and treatment studies.
Several biliary diseased models have been established in zebrafish, including Alagille syndrome [ 115], arthrogryposis-renal dysfunction-cholestasis syndrome [ 116], intrahepatic BA [ 117], and choledochal cysts [ 118].
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com