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Thirdly, a directly interaction between MdERF2 and MdERF3 restrain the binding of MdERF3 to the MdACS promoter, and then suppress the MdACS expressed in fruit flesh (Li et al. 2016).
To test for a directly interaction between Vpr and proteasomes, a plasmid pSF173 carrying a HA-Vpr fusion was transfected into a wild type fission yeast strain and co-immunoprecipitation analysis was carried out with anti-HA antibodies.
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Many studies have examined effect modification using stratified methods [ 12, 19, 20], which will decrease the statistical power and lack the ability to examine directly interaction effect.
Although R372 does not directly hydrogen bond to the substrate, except for a water-mediated interaction, it directly interacts with D345 and E351, which binds Ca2 and is close to the active site residue D350.
These gene products directly interact in a protein interaction network (Supplementary Figure 7B), suggesting functional associations.
To address this challenge, we developed a high-throughput reverse engineering strategy to analyze how thousands of specific perturbations (encompassing all point mutations in a central gene) impact both a specific edge (interaction to a directly connected node) and an overall network function.
This limitation also precluded our ability to examine more directly a potential interaction between lead in drinking water and water fluoridation method that would be expected if the hypothesized enhancement of lead uptake were correct.
It appears that this effect is not simply the result of C17,20-lyase C17,20-lyaseeductand/ortivity but is also mediated through a negative interaction directly with the androgen receptor (Long et al, 2001).
We chose single-molecule fluorescence spectroscopy as a way of directly assessing interactions between cavin proteins in complex mixtures.
It is interesting to note that UVR8 is the only photoreceptor to show a light-dependent interaction directly with COP1, independent of COP1 accessory SPA proteins [ 1, 21].
Resting-state functional magnetic resonance imaging (fMRI) approaches, which reveal patterns of coherent spontaneous fluctuations in the fMRI signal, offer a means to quantify directly functional interactions between the hemispheres.
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