Colour variation of the grape berry conforms to a peculiar pattern of genotype-specific expression of the whole set of anthocyanin genes in a direct transcript-metabolite-phenotype relationship.
The results showed that Tcf4 specifically bound to both amplicons in the miR-181a-2/miR-181b-2 promoter (findicating indicathat thet the miR-181a-2/miR-181b-2 transcript is a direct transcriptional target of Wnt/β-catenin canonical signaling pathway in HCC.
Data on gene expression of S. aureus during colonization is limited to the direct transcript analysis of a few genes [ 23, 24].
Knockdown of FMR4 by several siRNAs did not affect FMR1 expression, nor vice versa, suggesting that FMR4 is not a direct regulatory transcript for FMR1.
In contrast, the DGE protocol is more suitable and affordable for comparative gene expression studies because it enables direct transcript profiling without compromise and potential bias, thus allowing for a more sensitive and accurate profiling of the transcriptome that more closely resembles the biology of the cell [ 9, 13- 17].
So far, direct transcript analysis has been performed during different kinds of infection [11], [18], [41], [42], [43], [44], [45].
This has led to statistically relevant and high quality data, which we independently verified using direct transcript level measurements.
However, the emergence of next-generation-sequencing (NGS) with its direct transcript identification, lower cost, larger dynamic range and superior detection of low abundance genes [ 10- 12], is making microarrays obsolete.
Additionally, the fact that 20 out of 50 correspond to transcripts encoding ribosomal proteins might indicate that a direct correlation between transcript levels and protein abundance would be a general rule in Leishmania.
He served as a consultant on the new film, which follows his original study in detail, relying on direct transcripts from the experimental recordings and taking few dramatic liberties.
The testimony and other material alone, it was said, included thirty-two bofks of direct transcripts, six books of supplemental documents and affidavits, and volumes of maps, charts, exhibits, and internal documents.
