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The classic method for producing DME is by dehydration of methanol in a catalytic gas-phase reactor, and purification in a direct sequence of two distillation columns.
In this study, a direct sequence of distillation columns was studied and the separation system is different from the previous studies.
Conventionally, high purity DME is synthesized by dehydration of methanol produced from syngas, in a process involving a catalytic fixed-bed reactor and a direct sequence of two distillation columns.
and is guaranteed to converge to the point in the intersection of the convex sets specified by provided for all 1, 2, 3 regardless of the initialization that is a direct sequence of the fundamental theorem of POCS [9, page 1066].
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This was followed by a direct sequence analysis of the amplified products.
The expected amplification product of 277 bp was used for HBV genotyping by means of a direct sequencing analysis using an automated sequencer (ABI Prism 3100, Applied Biosystems, USA).
The complete genome sequence was determined by a direct sequencing using the products of RT-PCR, 5'RACE, and 3'RACE as sequencing templates [ 10].
We therefore adopted a direct sequencing approach complemented by cloning of selected accessions.
As a consequence direct sequencing of L2R3 PCR products from crude genomic DNA of VL species could not be performed, thus A2 sequences were obtained from clone libraries of L. infantum MHOM/FR/92/LEM2385-clone 1.
A TaKaRa One-Step RT-PCR Kit (TaKaRa, Dalian, China) was used for amplification of region C and region A, and direct sequencing of PCR products was carried out by TaKaRa Biotechnology (Dalian, China).
Because concerted evolution has generally homogenized sequence variation among the numerous ribosomal DNA copies within an individual, direct sequencing of this region is possible for most systems.
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