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This assumption is tested by a direct orientation measurement using Electron Back Scattering Patterns (EBSP).
The last two types were joining of two segments in a direct orientation, accounting for 15% of the total chimeras.
CDSs in TGAs are principally in a direct orientation (78.9%) with approximately the same proportion of sense and antisense orientations.
On this contig, the nematogalectin-related gene has a direct orientation and is followed by the nematogallectin A gene which has the same orientation.
Sequencing of the 5S 18S E. nebrodensis clone showed the presence of a functional 5S gene in a direct orientation as in Ginkgo.
All three test probes (TUPLE1, b186O8 and p901P22) were clearly triplicated, demonstrating a duplication in 22q11.2 with a direct orientation, corresponding to the longer (3 Mb) of the two recurrent CNVs observed in this region [ 26].
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While A starts with a gene in direct orientation, B starts with a gene in reverse orientation.
The pGIN2 clone isolated by us harboured a 5S insertion in direct orientation located downstream from the 26S gene.
To test the recombination activity of mutant phiC31 integrases in vivo, the wild-type or mutant plasmid containing an ampicillin resistance gene, and the report plasmid pBCPB+ with attP and attB sites in direct orientation flanking a lacZ gene on a chloramphenicol resistant, were co-transformed into an E. coli strain Top10.
The plasmid pCMVInt, which expresses phiC31 integrase in mammalian cells under control of the cytomegalovirus (CMV) immediate-early promoter, and the pBCPB+ plasmid, which carries the phiC31 recognition site attP and attB in direct orientation flanking a lacZ gene on a chloramphenicol resistant ColE1 derivative, were a kind gift from Dr. M.P. Calos.
Separating them are the following three genetic components: (1) two LoxP sites in direct orientation with a multiple cloning site in between them; (2) the LoxP cassette is followed by a single FRT site; (3) the mini-yellow transformation marker completes piB-LLFY(BI).
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