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In all patients there was a direct heart cannulation with the venous cannula placed in the right atrium, an additional venous cannula of smaller size in the left atrium, and the arterial cannula placed in the ascending aorta.
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Direct heart compression seemed infeasible because of firmly adhesive right lung and mediastinum.
Nonlethal mechanical trauma was characterized by the 100% survival rate during the first 24 h after trauma, the lack of circulatory shock and without direct heart injury.
Blood was sampled by direct heart puncture.
Blood was collected by direct heart puncture method.
For large animal experiments, blood was obtained from pigs by direct heart puncture under sterile conditions.
Venous blood samples were collected by direct heart puncture under deeply ketamine anaesthesia (Troy Laboratories, Australia).
Blood was collected via direct heart punctuation for renal function assay.
After 14 days, the animals were sacrificed under mild ether anesthesia, and the blood was collected by direct heart puncture method.
Although in vivo lineage tracing during murine cardiogenesis was able to indicate that a substantial portion of ECs in the mammalian heart are derived from the Isl1+ heart progenitors, a direct demonstration in human hearts via clonal assays was found difficult due to inefficient differentiation of the Isl1+ progenitors toward the endothelial lineage.
Artist and musician Laurie Anderson has directed Heart of a Dog, a feature film inspired by the life and death of her terrier Lolabelle, and dedicated to her late husband, Lou Reed.
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