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Automated annotation of proteins was based on a diminishing identity threshold scale for Blastp [ 66] matches against protein databases consisting of (1) the reference genome wPip, (2) other Wolbachia genomes, (3) swiss-prot and (4) the non-redundant database (NR).
Automatic annotation was carried out using BLASTp [ 49] in a hierarchical approach that prioritised a high-quality manually curated annotations by using a diminishing BLASTp identity thresholds against databases comprising proteins from i) Salmonella Typhi str.
Parameter sets with distances less than εx are accepted; a diminishing series of thresholds, ε1 > ε2 > ε3, etc, progressively focuses the search on those parameter values that best match the data.
It will arrive at a diminished world.
While it is clear that saturating levels of sequencing coverage are required for the lowest false positive and false negative rates in TGE experiments [ 6], it is also clear that there is a diminishing return after the threshold coverage level for variant detection is exceeded, and, in fact, over-coverage can introduce errors [ 7].
It is an anonymous newsstand with no plaque or notice, hidden in plain sight and recalled only by a diminishing few who collected their daily papers before crossing the Center's threshold.
There's a certain threshold of money needed to keep me content and after that, there is a diminishing return.
This is a diminishing asset".
"Heaven is not a diminishing field.
Vastness yields a diminishing return.
A diminishing trickle.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com