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The design of primers specific to a difficult sequence of telomeres is challenging and there are already numerous protocols for this application [ 19, 20].
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The battle in the nebula was a difficult sequence to accomplish without the aid of computer-generated models.
"Inside Deep Throat" offers few inklings of that persuasive grimness, except for a difficult sequence with Patsy Carroll, a school friend of Linda's, who saw the film, went home, and wept.
Forty-eight normal adults (24 females and 24 males) counted backward by ones or by twos while typing an easy or difficult sequence of letters with either hand.
This monolith was derived into Wang resin and used in the synthesis of a difficult sequence-acyl carrier protein fragment 65 74 (ACP 65 74) in a new designed continuous flow equipment.
The sequences became increasingly more difficult (from a sequence of two digits to a maximum of nine digits) until the children obtained the required number of errors for discontinuation.
Reconstruction of evolutionary history of bacteriophages is a difficult problem because of fast sequence drift and lack of omnipresent genes in phage genomes.
The length of the sequence started with two blocks, and became increasingly more difficult (to a maximum sequence of eight blocks) until they obtained the required number of errors for discontinuation.
A problem is difficult if an appropriate sequence of steps to solve it is not known.
Amdoparvovirus sequences, except for a difficult-to-sequence high guanine cytosine nucleotide content of 62-bp gap, were recovered, which are consistent with the sequences acquired by Sanger sequencing of viral DNA amplified from tissues.
For a few of the more difficult sequences, we used 5' or 3' RACE or RT-PCR to verify/complete the sequence.
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