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Mai pen rai is often a difficult expression to translate.
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We successfully expressed P14K through genetic modifications according to N-end rule and analyzed the mechanism for its difficult expression.
The strategy used for P14K expression in this study may be useful for the heterologous expression of other difficult expression proteins.
Furthermore, these strategies, which promote the over-expression of the instable P14K in E. coli, might also be helpful for the heterologous expression of other difficult expression proteins.
Our results are not only useful for clarification of the role of P14K involved in the NHase maturation, but also helpful for heterologous expression of other difficult expression proteins.
The original IPDE is written in relatively simple language and difficult expressions have been avoided.
Reasons for false negative errors included logical relations that were not modeled, and grammatically difficult expressions.
In this case, we tried to strike a difficult balance between enabling expression and protecting our community and ended up making a mistake".
However, the gp160 antigen, with its strong hydrophobic regions and heavy glycosylation, is a difficult candidate for heterologous expression and purification [ 8, 14, 15].
Regime change is an easy expression for a difficult job.
Controversy over Salman Rushdie's invitation to the Jaipur Literature Festival and the public reading of his "The Satanic Verses" has forced organizers to walk a difficult path between championing free expression and keeping the festival safe and open, William Dalrymple, one of the festival's co-directors, said Sunday.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com