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Analysis was carried out on doublets composed of two cell types each with a different staining of either DiD lipid dye for HL2/3 effector cells or Hoechst for TZM-bl target cells.
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In line with previous observations, the altered pattern of differentiation markers expressed in osteoblasts accompanied by a different staining pattern of mineralization and increased proliferation, suggests that LNCaP-19 cells stimulate an immature population of osteoblasts that contributes to the major part of the newly synthesized bone.
It is important to note that stains are usually not inert, but have different characteristics that can lead to different staining of the specimen (e.g., (18- 20)).
Significantly different staining of PPAR β was observed in the epithelium and stroma of benign and borderline tumours compared with grades 1, 2 and 3 tumours (χ=11.28, d.f.=4, P<0.05; χ=16.15, d.f.=4, P<0.005).
Additional file 3 shows other examples of different staining intensities of RANK.
Additional file 1 shows examples of different staining intensities of RANKL.
c Graft particles (EG) tightly integrated in newly formed mature bone (NB) or blue-stained osteoid (O); different staining characteristics of newly formed bone and recently formed bone (RB).
First, however, control experiments were performed to ascertain the viability of conidia that displayed different staining patterns of the neutral red dye.
The different staining intensities of NKp30-ILZ and NKp44-ILZ could either reflect a different expression of NKp30L and NKp44L, or be simply the result of a higher affinity between NKp44L and the NKp44 receptor.
Different staining patterns of AIB1 are shown in supplementary Figure S1, available at Annals of Oncology online.
With different staining patterns of HEp-2 cells.
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