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"You have to have a different breathing rhythm, a different backbone, a different weight.
It forms a similar structure to DNA, but with a different backbone and would've been simpler to produce and replicate on primordial Earth.
Apparently, the protein structure fluctuates within expected margins, but there is no indication for a systematic long-term trend to escape from the bilayer or to switch to a different backbone fold.
To further prove that the presence of the TFS is not necessary for relaxation, plasmids with a different backbone were subjected to TFO−C141S treatment.
This segment, however, adopts a different backbone structure in eNOS compared to iNOS and nNOS, and despite the relatively high mobility of this region, the polypeptide is closer to the conserved Tyr in eNOS, thus presenting a greater energy barrier to the conserved Tyr adopting the out-rotamer conformation.
Here, Oliveberg and co-workers observed that, while the wild-type S6T protein folded through a globally diffuse transition state that typified nucleation-condensation, a circular permutant (with conjoined wild-type termini and a different backbone cleavage site) exhibited an extremely polarized transition state [ 19].
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Modified oligonucleotides containing an abasic site and two different backbone modifications were used to further probe the role of residues K170, K194 and H198 in translocation (Table II; Figure 6C; Supplementary Figure S5).
An important extension was to investigate a different chiral backbone that had a strong heritage in asymmetric catalysis, 1-amino-2-indanol.
In addition, the two selections (in Grimm et al. [37] and in our study) were done in a different AAV backbone context.
In a parallel set of experiments using a different antibody backbone but the same three tag placements, we tested the stability of aldehyde-tagged HIPS conjugates in plasma at 37 °C.
To further confirm that this dual reporter configuration is useful in validating TALEs function, a new set of dual reporters were built with a different plasmid backbone, namely the minicircle plasmid DNA.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com