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A difference of one cycle was considered to represent a twofold change in gene expression.
Within this region of the amplification curve, a difference of one cycle is equivalent to doubling of the amplified PCR product.
Assuming the Ct value reflects the initial starting copy and there is 100% efficiency, a difference of one cycle is equivalent to a 2-fold difference in starting copy.
Assuming that the Ct value is reflective of the initial template amount (copy number) and that there is 100% efficiency, a difference of one cycle is equivalent to a 2-fold difference in initial copy number (Walker 2001).
Within this region of the amplification curve, a difference of one cycle is equivalent to doubling the amplified PCR product.
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Within this region of the amplification curve, each difference of one cycle is equivalent to a doubling of the amplified product of the PCR.
The larval samples that were geographically close and had a difference of one complete life cycle stage between the population groups were subjected to molecular studies to evaluate their relatedness.
At first sight a difference of one step may not seem significant, but as cycles become shorter the bites become progressively larger.
"A difference of one 12-oz.
Power calculations (Fig. 1) show that for a read depth of 50 and a 67 33 allelic imbalance, which corresponds to an average of one cycle difference in a qPCR experiment between individuals homozygous at both alleles (a two fold difference), the probability to observe a P-value more significant than 0.001 is 19% (Fig. 1).
Under optimal conditions, a two-fold difference in the number of templates amplified results in a shift of one cycle between two CT determinations.
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