In order to better define the genomic structure of the sheep TRA/TRD locus, the determined sequence of the chromosome 7 genomic scaffold was aligned with the human counterpart, using the PipMaker program, and the alignment expressed as a dot-plot sequence comparison graph (Fig. 5).
Using energy from an air-driven piston to turn a wheel, the device advances a conveyor belt with a pre-determined sequence of stimuli that differ in their spatial features into the tunnel of the MRI.
Each study center had a pre-determined sequence of randomization numbers which were allocated sequentially to eligible subjects.
The fusion of a known adaptor of determined sequence to the 5'-end of an AD (or other short site-dependent primer) is the main characteristic of FPNI-PCR, and it is this trait which differentiates FPNI-PCR from TAIL-PCR.
The basic principle of FPNI-PCR is outlined in Figure 1 and 2. The method centers on a series of primers encompassing sequence-specific primers (designed on regions of known DNA sequence), and fusion primers which contain an arbitrary degenerate (AD) section fused to a section of determined sequence (fusion primers).
We concatenated newly-determined sequences of Kuchinoshima-Ushi and the published sequences and aligned them for the phylogenetic analysis.
Thanks to the development of artificial nucleases capable of generating DNA double-strand breaks (DSBs) in pre-determined sequences of the genome (Porteus & Baltimore, 2003; Urnov et al, 2010; Cong et al, 2013; Joung & Sander, 2013), targeted gene therapy is entering into the clinics (Tebas et al, 2014).
For the DSE, an absence of determined sequence motif is consistent with previous experiments showing that point mutations in this region had no effect on polyadenylation efficiency [ 11].
In the structured approach, the interviewer must faithfully asks in a pre-determined sequence, a series of closed pre-defined questions, corresponding to the diagnostic criteria.
By integrating several product types into a product family (a "block") and by scheduling them block-wise in a pre-determined sequence, the complexity of the model is significantly reduced.
A "window of analysis" of pre-determined sequence length of 1500 nucleotides that includes the full length of the targeted exon (see Methods and Materials) corresponds to a "step of transcriptional analysis".
