The amount of lipase produced in the designated medium was in agreement with that of predicted values by statistical method.
Growth medium containing 5.5% FBS and 20 mM HEPES was designated medium A. CT60 cells were plated in Falcon 12-well plates (VWR International, Inc., Bridgeport, NJ) in growth medium with 10% FBS, and after 24 h 10 µM of compound was added.
Each well was then topped up with their designated medium (3x scaffolds were each soaked in DMEM, αMEM, supplemented MG63 media, and PBS).
Insulin infusion was constant on each day, at one of three randomly assigned rates: 1) a minimum rate of either 0.01 units/kg/h or an average of the subject's daytime basal rate, whichever was higher (designated "low"); 2) a rate of 0.05 units/kg/h (designated "high"); and 3) midway between the minimum and maximum rates (designated "medium").
The bonds will be designated medium-term notes.
CDE medium containing 2%% maltose as a carbon source (designated CDEm medium) or YPM medium (1 % yeast extract, 2%% polypeptone, 2%% maltose) was used for up-regulation of glaA142 promoter-driven ustR in the ustR EX strains.
Leishmania major Friedlin (MHOM/JL/80/Friedlin) were grown as promastigotes in modified Eagle's medium (designated HOMEM medium) supplemented with 10% (v/v) heat-inactivated fetal calf serum (FCS) at 25°C as described previously (Besteiro et al., 2006).
Leishmania major Friedlin (MHOM/JL/80/Friedlin) were grown as promastigotes in modified Eagle's medium (designated HOMEM medium) supplemented with 10% heat-inactivated fetal calf serum (FCS) at 25°C as described previously (Besteiro et al., 2006).
This medium was designated Granada Medium (GM).
L. major (MHOM/JL/80/Friedlin) promastigotes were grown in modified Eagle's medium (designated complete HOMEM medium) with 10% (v/v) heat-inactivated foetal calf serum at 25°C as described previously [ 71].
The first type, designated Granada Medium (GM) [ 15], is an adaptation of Islam's medium [ 16], and exploits the ability of GBS to synthesize - under anaerobic conditions and on media containing starch and serum - an orange pigment, recently identified as granadaene [ 17].
