Glycans were purchased from Dextra Laboratories (Reading, UK) and Glycoseparations (Moscow, Russia), and functionalised with an amine group using a modification of a previously described methodology by Vetter and Gallop [58].
In order to differentiate between the two intracellular pools of SopB; (i) translocated and (ii) intrabacterial, we used a recently described methodology based upon a 13-amino-acid tag derived from glycogen synthase kinase 3β (GSK) (Garcia et al., 2006).
The process of analysis was based on a phenomenological approach according to a previously described methodology.
These sources were screened for a well described methodology, accurate statistical analysis and an adequate sample size where relevant.
and Mathcad 13 (Mathsoft Engineering and Education, Inc .. Changes in the complete spectra obtained using the Olis RSM as a function of time were analyzed using a previously described methodology.
The content of total phenolic compounds (free and conjugated) expressed as mg quercetin equivalents (QE) was determined according to a previously described methodology [35].
According to a previously described methodology [13, 16], skeletal muscles were evaluated by US scan, collecting both quantitative and qualitative data.
For these reasons, in our study, we have designed an experimental animal model with BMX in rats for the in-depth analysis of bone repair process and used a previously described methodology albeit safe and effective.
In the last part of our study, we have adapted a previously described methodology [22] that utilized SPR technology to determine dissociation constants (KD) of selected antibodies.
The MaR derived from T2-weighted imaging was assessed according to a previously described methodology.
