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When cultured in 7.5%/7.5% of NH4+/PO42−, C. reinhardtii showed a 2.4-fold increase in TAG production with a 3.5-fold increase in biomass compared to level obtained under an only NH4+ depleted condition.
Kid depletion restored a monophasic elongation of the spindle in each individual Clasp depleted condition (Fig. 2A,B; supplementary material Fig. S1F).
This oscillating pattern of gene regulation may reflect a homeostatic control mechanism that is gradually adjusting the gill to the new zinc depleted condition.
The majority (72%) of the 29 regulated transcription factors were down-regulated under the zinc depleted condition.
The Hsp40 family protein Ydj1 has an LC domain (Table S7), binds Q/N-prion domain proteins (Summers et al. 2009), and in our proteomic studies associates with Dhh1−GFP only in the glucose depleted condition (Table 2 and Table S5).
A temporal profile of the overall numbers of up- or down-regulated transcripts showed that changes in gene expression occurred as early as 8 hours following introduction of the zinc depleted condition, reached the peak level at Day 7, and then subsided by Day 14.
A positive value represents an induction of gene expression under iron depleted conditions, and a negative value represents a repression of gene expression under iron depleted conditions.
To test the importance of iron acquisition genes under iron depleted conditions, a putative alcaligin biosynthesis protein SO3032 was selected for examination.
The spores were vortexed and concentrations of spores were determined by counting with a hemacytometer. A. fumigatus was grown in a liquid shaker under iron depleted conditions.
In a recent study, Blatzer et al. [ 37] performed gene expression analysis under iron replete and iron depleted conditions of a number of genes involved in iron homeostasis using Northern blots.
A significant portion of the genes differentially regulated in iron depleted conditions do not have a known function (Figure 4, gray slice).
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