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The integrity of the mRNA was confirmed by electrophoresis in a denaturing 1 % agarose gel.
After purifying the linear PCR products, the samples where electrophoresed through a denaturing 10% polyacrylamide gel.
About 200 µg of the total pooled RNAs were separated onto a denaturing 15% polyacrylamide gel.
The reaction products were separated on a denaturing 10% acrylamide sequencing gel, and exposed to a PhosphorImager screen.
ODNs were purchased from Eurogentec (Liege, Belgium) and further purified by electrophoresis in a denaturing 18% acrylamide/urea gel.
PCR products were separated on a denaturing 6% polyacrylamide gel.
(A ) Denaturing immunoprecipitation (IP) of BMH crosslinking reactions.
Total RNA was separated on a denaturing 1.5% formaldehyde agarose gel.
Proteins (15 μg) from each lysate were run on a denaturing SDS/4 20% PAGE gel.
The sRNAs were then separated on a denaturing 12,5% polyacrylamide (PAA) gel.
Digestion and modification products were fractionated on a denaturing 8% polyacrylamide gel.
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