Figure 10 shows an example set of search results when searching for a particular biological process term in the demonstration database.
Ebrary.com already has more than 130,000 volumes in its demonstration database and says that it may include as many as 600,000 by the time it opens in the fall.
Demonstration database files are also provided to assist in illustrating how the LRE Analyzer functions, along with insights into the capabilities of LRE quantification.
The LRE Analyzer can be installed by downloading the files provided at the open source project website (http://code.google.com/p/lreqpcr), which also includes demonstration database files.
Data presented in the demonstration databases were generated using an Applied Biosystems 7500 instrument (normal ramping), Qiagen QuantiTect in a 10 µl reaction volume containing 500 nM of primers, in 96 well white plates (ABgene) sealed with MicroAmp film (Applied Biosystems), and amplified using a cycling regime of 15 min activation at 95°C, followed by 50 cycles of 95°C −10 s, 65°C −120 s.
I am indebted to Don Stewart for conducting the qPCR analysis contained within demonstration databases, to Michel Cusson for reviewing the manuscript, and I thank Pamela Cheers for editorial assistance.
Generated during a study investigating the reproducibility of LRE quantification for gene expression profiling, the datasets provided in the demonstration databases focus on assessing the reproducibility of reverse transcription and run-to-run quantitative variances.
The panel session closed with a demonstration of the Festus Lexicon database.
As a demonstration, using the company's employee database, a video screen displayed a live feed of a busy intersection nearby.
We provide a Web interface to perform queries on the database only as a demonstration of what MIRIAM Web Services can offer.
Indeed, when the police admit that someone could end up on a secret police database merely for attending a demonstration, it is exactly the time to worry.
