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In addition to RNA isolated from visually unaffected and necrotic tissues, RNA from the demarcation zone (between infected and non-infected tissue), and from nose and trachea originating from the same infection study were included in the RT-qPCR studies.
RT-qPCR, on the other hand provides additional, complementing results for the demarcation zone as well as a control sample group.
No significant difference of the miR-15a expression has been detected between the infected samples (visually unaffected, demarcation zone, necrotic), which is also reflected in the RNAseq results.
Lung samples were taken from three different sites: necrotic area, demarcation zone, and visually unaffected area.
There are no fences or demarcation zones to indicate areas where poaching is controlled.
Many residents along Corniche Mazraa, a major highway that has become a demarcation line between the factions, were seen leaving their houses for safer areas.
Simpson's arrival represented a demarcation point between an era of swaggering, scandal-plagued success, and the sobering realities of unprecedented and dispiriting toil for the fallen club.
Russian Foreign Minister Sergei Lavrov said on Friday agreeing a demarcation line between the rival sides was the crucial element that had stalled earlier truce efforts.
Participants constructed a demarcation line between the professional and the private context.
For early pilgrims, high tide also created a demarcation between the everyday and spiritual worlds.
The diagonal plate-dividing seam boardwalk serves as a demarcation between two eras of Chicago's landscape development.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com