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Chromosomes on slide preparations were denatured for 10 seconds in 70%% formamide/2x SSC at 72°C, followed by a dehydration in 70 %, 80%, 988 % ethanol bath series.
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After a progressive dehydration in a series ethanol concentrations up to 70%, samples were cleared in 8: 1: 2 chloral hydrate: glycerol: water.
Differentiation was achieved by 2 washes with diluted acetic acid followed by a short dehydration in a series of graded alcohols.
Seeking to meet the demand, Cargill devised a plan, which is still under consideration, to build a dehydration factory in El Salvador that would convert Brazilian ethanol for use in American automobiles.
Then, after a dehydration bake in an oven for 30 min, an SiO2 layer with a thickness of ~ 1 μm was formed on the silicon using the dry-wet-dry oxidation technique in a oxidation furnace (Tempress, Netherlands).
Mouse femurs were isolated, cleaned and immersed in PBS/2% paraformaldehyde for 6 h at 4 °C, followed by a dehydration step in 30% sucrose for 72 h at 4 °C.
The above results demonstrated that suppression of OsVPE3 caused decreased leaf width and guard cell length, promoting a dehydration tolerance in rice leaves.
Although serum bicarbonate is not the gold standard for dehydration, this study provides further evidence for the usefulness of the CDS as a dehydration marker in children.
In situ stained embryos were further processed by a serial dehydration in ethanol, followed by rehydration into 1×PBS.
A stepwise dehydration in ethanol was performed using 30 minutes incubations in 70%, 80 %, 90, and twice in 96% ethandl.
Cholera, although it can kill an adult through dehydration in half a day, is easily treated.
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