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MCT1 is also present on the membranes of erythrocytes, contributing a degree of staining within the lamina propria.
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As a consequence, the vast majority of cases produced a strong degree of staining intensity, which resulted in reduced variation of the staining intensity between the cases.
This was the only IL-2 receptor that showed a certain degree of staining intensity in fibrocystic lesions (Fig. 4b).
In fact, we observed worse RFS in patients with higher staining for Ki-67 compared with a lower degree of staining.
Whereas, strong staining in positive K562 cells and an absence of staining in the negative MDAMB231 cells was evident in the MCT1 assay, a degree of background staining particularly in the negative K562 cells was observed with the MCT4 assay.
Immunohistochemically, there was a difference in the degree of staining for HMB-45 between primary and metastatic lesion.
This latter measure is shown on the x-axis revealing subtleties of the variation of the ratio as a function of the overall degree of staining.
Within the ER α-negative group, there was a positive correlation between the degree of staining for AGR2 (>1% carcinoma cells stained) and the degree of staining for PgR (n=47 cases; Spearman's correlation coefficient 0.48; P=0.480.48
To assess the average degree of staining within a tumour, multiple regions were analyzed, and at least 100 tumour cells were assessed.
For each localization, an assessment was made as the degree of staining intensity and the percentage of cells stained with that intensity in the nucleus or in the cytoplasm, which was graded semiquantitatively to produce an intensity distribution score (IDS), with the maximum score 300 (if 100%% cells stain strongly positive) and the minimum score 0 (if 100%% cells stain negative).
Immunoslides were assessed in a microscope by counting of positive cells and degree of staining.
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