Thus, our null hypothesis for each tested phenotype was that benzoate degrading condition doesn't show a significant difference vs succinate degrading condition in the average expression of genes/proteins representing the phenotype.
Similar assumption can be made for p-coumarate degrading condition when it is compared with succinate degradation.
The conditions are referred as the p-coumarate degrading condition (Cou vs Suc) and the benzoate degrading condition (Ben vs Suc).
Benzoate degrading conditions are also characterized by presence of nitrogen fixation and formate utilization phenotypes in addition to phenotypes listed for p-coumarate degrading condition.
This may explain the lack of N2-fixation subpopulation under the p-coumarate degrading condition, although this subpopulation is found under the benzoate degrading condition.
Surprisingly, the benzoate degrading condition is also characterized by statistically significant activation of nitrogen fixation phenotype (p = 0,000053) and by formate utilization phenotype (p = 0.036).
Both routes of oxidation, however, have consistently greater up-regulation at the level of genes and proteins under p-coumarate degrading condition.
Therefore, the size of N2 fixing subpopulation in the benzoate degrading condition may be actually similar to the size of carbon dioxide utilizing subpopulation or greater.
In conclusion, the phenotype-based screening of gene expression and protein abundance under p-coumarate and benzoate degrading conditions versus succinate utilization condition shows that p-coumarate degrading condition is characterized by presence of 5 phenotypes: benzoate utilization, β-oxidation, non-β-oxidation, CO2 utilization, and H2 utilization.
As a young woman she had caught the attention of a British ship's doctor named Dunlop, who deceived her into following him to England, where he displayed her, in the most degrading conditions, as a sideshow curiosity.
