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While poxvirus early transcripts have a defined termination signal, late transcripts have heterogeneous 3' ends as the early termination signal is not recognized by the viral late transcriptional apparatus.
Polycistronic RNA initiating from H1 starts upstream of MT-TF, the gene encoding mt-tRNAPhe and has a defined termination site within MT-TL1 (mt-tRNALeu UUR)).
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The RP495-RP496 gene pair exhibited a similar termination profile: RP495 transcripts exhibit no defined termination site (Fig. 1C, Lane 2) and the probe specific for RP496 was fully protected (Fig. 1C, Lane 4).
If defined termination sites exist, we would expect to detect a spot of greater intensity on the 'Y-arc,' corresponding to paused replication forks.
Promoters of this type are preferred because they naturally direct the synthesis of small, highly abundant non-coding RNA transcripts, with defined termination sequences consisting of 4 5 thymidines (Ts) and have no requirement for downstream promoter elements [ 22- 24].
All animal studies should have clearly defined termination criteria, also called "humane endpoints", at which point animal suffering is no longer justified by the scientific value the animal is providing.
Recent studies, however, have shown that the distance from the PTC to various cues within the 3' UTR (particularly the cytoplasmic poly-A binding protein PABP) can also play an important role in defining termination events as aberrant [ 3, 8- 13].
However, in the case of rho-independent terminators, the O/E ratio of the 6,079 novel transcribed regions was not significant (Additional File 3B), suggesting that most of the novel transcribed regions may not have a defined transcription termination site or may lack the 3' sequence.
We suggest that this change of growth morphology is due to increased selective etching reactions occurring concurrent with the growth reactions which might also contribute to a less defined h-BN termination.
Neonatal death is defined as termination of life of a live-born child within 28 days of life.
During our experimentation, we defined the termination criterion that the mining process did not exceed 10 days of running time.
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