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An angler purchases the right to fish a defined stretch of stream for a numbered set of hours, called a beat.
A defined stretch of 60 cm was chosen for filming and analysis.
This created labelled cDNA fragments comprising the most 3' located site of each restriction enzyme, respectively, towards a defined stretch of the poly-A tail of the cDNA, a defined part of the cloning vector and the labelled oligonucleotide.
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This could result in, for example, the insertion of a short defined stretch of amino acids into a particular site of a protein, an epitope tag fusing in frame with the targeted protein for endogenous tagging, or a recombination site enabling further site-specific engineering.
One subsection where relatively tall new condo buildings did take root, to an extent, is Greenwood Heights, an imprecisely defined stretch at Sunset Park's northern end, between Fourth Avenue and Green-Wood Cemetery. Green-Wood Cemetery
These results suggested that deoxygenation-activated elevation of [Ca2+]I in SS cells required both HbS polymerization and activation of a (pharmacologically defined) stretch-activated ion conductance.
Nearly a third of the way through the season and already playing catch-up to the eighth-place Milwaukee Bucks, the Knicks face a defining stretch.
The usefulness of this method was demonstrated by means of two frequently occurring problems: the cloning of defined stretches of Poly-Q encoding sequences and the generation of polyadenine sequences for in vitro transcription of polyadenylated mRNA.
This means involving the right people, defining stretch goals, marshaling resources, setting deadlines, pushing for results, and then rewarding successes.
The resulting cDNA pool containing a defined poly A stretch of 16 nucleotides was cloned uni-directionally in the vector, a cDNA library was constructed in E. coli and plasmid DNA was isolated from the resulting colonies.
To obtain a load of 50-60% of MVC the green Thera-band® was stretched of a defined% of the initial length: for lateral abduction the patient was instructed to stretch the Thera-band® of about 50% of the band initial length, for biceps flexion and triceps extension of about 25%.
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