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MPSS provides a comprehensive assessment of gene expression by generating short sequence tags, each 20 bp long, produced from a defined position for each transcript.
Several other methods were developed based on the principles of SAGE (LongSAGE, superSAGE and 5′ SAGE) methods that sequence a small fragment of cDNA from a defined position for expression profiling [ 23– 25].
For example, microchambers for holding C. elegans in a defined position for imaging and laser microsurgery have been developed and used for screens of factors involved in neuronal regeneration 27.
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Individuals are more likely to accept a job if the overall WTP for a defined position is positive.
We overcame this bias using Adaptive Focussed Acoustics from Covaris to shear cDNA and using a specific adapter, we could sequence cDNA tags from a defined position in a transcript for expression profiling.
The majority of genes with antisense transcription had a clear peak of tags at a defined position suggesting a relatively defined polyadenylation position for these transcripts, supporting a possible regulatory role for these antisense transcripts.
Also BACs generated by random cloning techniques could be adapted subsequently by transposition of the mini-F vector to a defined position to tweak those clones for further work [45].
The rod, representing the weight-bearing line, should pass through the knee joint at the preoperatively defined position for the mechanical axis.
Their ideas were organized around the following ideas: (i) creating defined positions for students with specific responsibilities within a logical organizational structure, (ii) providing a diverse array of opportunities for students, (iii) empowering student leaders with specific and meaningful responsibilities, and (iv) implementing steps to make students feel appreciated, respected, and valued.
This helped to define positions for substitution, which could lead to a greater thermostability without losing or diminishing enzyme activity.
The key feature for our purposes is the consistent incorporation of a user defined position dependent energy contribution for each nucleotide that remains unpaired.
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CEO of Professional Science Editing for Scientists @ prosciediting.com